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bp502424  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology bp502424
    Bp502424, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bp502424/BioPORTER+Protein+Delivery+Reagent+QuikEase+Kit/pm32220302-566-20-17
    Average 96 stars, based on 6 article reviews
    bp502424 - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Structure and Function of the Bacterial Protein Toxin Phenomycin.
    Article Snippet: Intracellular delivery of phenomycin into living cells was performed using the BioPORTER Protein Delivery Reagent QuickEase Kit (AMSBiotechnology (Europe), Cat#: BP502424).

    Virus:

    Article Title: Structure and Function of the Bacterial Protein Toxin Phenomycin.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains DH5a NEB Cat# C2989K BL21 (DE3) NEB Cat# C2527I LOBSTER Andersen et al., 2013 N/A Biological samples Cell pellet from Thermus Thermophilus HB27 BFF, Athens-Gerorgia, USA https://bcmb.franklin.uga.edu/bff N/A Wheat germ Sigma Aldrich Cat# W0125 Chemicals, Peptides, and Recombinant Proteins HisTrapTM Excel column GE Healthcare Cat# GE17-3712-05 Amicon Ultra-4 spin columns Sigma Aldrich Cat# UFC800308 C4 column Vydac Cat# 214TP510 SA matrix Bruker Cat# 8201345 Tris(3- hydroxypropyltriazolylmethyl)amine (THPTA) Sigma Aldrich Cat# 762342 Sulpho-Cy5 azide Lumiprobe Cat# A3330 FAM-azide Lumiprobe Cat# C4130 Azide-PEG3-biotin Sigma Aldrich Cat# 762024 (+)-Sodium L-ascorbate Sigma Aldrich Cat# A7631 Nunc Lab-Tek 8-well chambered coverglass Thermo Scientific Cat# 145411 Poly-D-lysine hydrobromide Sigma Aldrich Cat# P7405 Hoechst 33342 Thermo Scientific Cat# 62249 Lipofectamine 3000 Reagent Kit Thermo Scientific Cat# L3000001 Transferrin Alexa fluor 555 conjugate Invitrogen Cat# T35352 FBS Gibco Cat# A3160802 Dip and Read Streptavidin sensors FortéBio Cat#18-5019 Zeba Spin Desalting Columns, 7 kDa cut-off Thermo Scientific Cat# 89890 MitoTracker Deep Red Invitrogen Cat# M22426 Wheat-germ agglutinin/Alexa Fluor 555 conjugate Invitrogen Cat# W32464 Concanavalin A/Alexa Fluor 488 conjugate Invitrogen Cat# C11252 Phalloidin/Alexa Fluor 568 Invitrogen Cat# A12380 SYTO 14 green fluorescent nucleic acid stain Invitrogen Cat# S7576 Hoechst 33342 Invitrogen Cat# H3570 Bacterial ribosome This paper N/A Yeast ribosome This paper N/A Human ribosome This paper N/A Plant ribosome This paper N/A Critical Commercial Assays CellTiter Blue Promega Cat# G8080 BioPORTER Protein Delivery Reagent QuickEase Kit AMS Biotechnology (Europe) Cat# BP502424 Deposited Data Morphological profiles and correlation matrix This paper Mendeley Data: https://doi.org/10.17632/ 772b973hhz.1 Phenomycin NMR structure This paper BMRB: 50088 Phenomycin structure This paper PDB: 6TKT (Continued on next page) Structure 28, 1–12.e1–e9, May 5, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines Human: U2OS cell line (taken from 15-year-old female) ATCC ATCC HTB-96; RRID:CVCL_0042 Human: MCF7 (taken from 69-year-old female) ATCC ATCC HTB-22; RRID:CVCL_0031 Human: PANC-1 (taken from 56-year-old male) Cell Line Services (CLS) CLS 300228; RRID:CVCL_0480 Human: BxPC3 (taken from 61-year-old female) Sigma Sigma 93120816; RRID:CVCL_0186 Human: HCC827 (taken from a 39-year-old female) ATCC ATCC CRL-2868; RRID:CVCL_2063 Human: MDA-MB-231 (taken from a 51-year-old female) Cell Line Services (CLS) CLS 300275; RRID:CVCL_0062 Human: A549 (taken from a 58-year-old male) Sigma Sigma 86012804; RRID:CVCL_0023 Human: SiHa (taken from a 55-year-old female) ATCC ATCC HTB-35; RRID:CVCL_0032 Human: HaCat (taken from a 62-year-old male) Creative Bioarray CSC-C8977H; RRID:CVCL_0038 Mouse: AML12 (taken from male) ATCC ATCC CRL-2254; RRID:CVCL_0140 Human: FreeStyleTM HEK-293F (taken from female embryo) Thermo Thermo R79007; RRID:CVCL_6642 Experimental Models: Organisms, Strains S. Cerevisiae - Dstm1: (strain JD1559) J. Dinman, (University of Maryland) N/A Oligonucleotides Fwd K69A CCTGCAAAGGATGCGAAGAGTGATATG This paper N/A Rev K69A CATATCACTCTTCGCATCCTTGCAGG This paper N/A Fwd M3C phenomycin CTTTATTTTCAGGGCGCCTGTGCGAA TCCAAAGACCATC This paper N/A Rev M3C phenomycin GATGGTCTTTGGATTCGCACAGGCGC CCTGAAAATAAAG This paper N/A Fwd M3C aquimarinamycin CTTTATTTTCAGGGCGCCTGTGGACCC ATTAAGGATCGTGAC This paper N/A Rev M3C aquimarinamycin GATCCTTAATGGGTCCACAGGCGCCCT GAAAATAAAGATTC This paper N/A Fwd M3C carotamycin CTTTATTTTCAGGGCGCCTGTGCTATTA GCGATAATGACTACAACCG This paper N/A Rev M3C carotamycin CATTATCGCTAATAGCACAGGCGCCCTG AAAATAAAGATTCTC This paper N/A Recombinant DNA pETM11 EMBL G€unter Stier GFP-Rab7 Choudhury et al., 2002 Addgene plasmid # 12605 Addgene_12605 KSH_AAA (see Table S3) This paper N/A (Continued on next page) e2 Structure 28, 1–12.e1–e9, May 5, 2020

    Recombinant:

    Article Title: Structure and Function of the Bacterial Protein Toxin Phenomycin.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains DH5a NEB Cat# C2989K BL21 (DE3) NEB Cat# C2527I LOBSTER Andersen et al., 2013 N/A Biological samples Cell pellet from Thermus Thermophilus HB27 BFF, Athens-Gerorgia, USA https://bcmb.franklin.uga.edu/bff N/A Wheat germ Sigma Aldrich Cat# W0125 Chemicals, Peptides, and Recombinant Proteins HisTrapTM Excel column GE Healthcare Cat# GE17-3712-05 Amicon Ultra-4 spin columns Sigma Aldrich Cat# UFC800308 C4 column Vydac Cat# 214TP510 SA matrix Bruker Cat# 8201345 Tris(3- hydroxypropyltriazolylmethyl)amine (THPTA) Sigma Aldrich Cat# 762342 Sulpho-Cy5 azide Lumiprobe Cat# A3330 FAM-azide Lumiprobe Cat# C4130 Azide-PEG3-biotin Sigma Aldrich Cat# 762024 (+)-Sodium L-ascorbate Sigma Aldrich Cat# A7631 Nunc Lab-Tek 8-well chambered coverglass Thermo Scientific Cat# 145411 Poly-D-lysine hydrobromide Sigma Aldrich Cat# P7405 Hoechst 33342 Thermo Scientific Cat# 62249 Lipofectamine 3000 Reagent Kit Thermo Scientific Cat# L3000001 Transferrin Alexa fluor 555 conjugate Invitrogen Cat# T35352 FBS Gibco Cat# A3160802 Dip and Read Streptavidin sensors FortéBio Cat#18-5019 Zeba Spin Desalting Columns, 7 kDa cut-off Thermo Scientific Cat# 89890 MitoTracker Deep Red Invitrogen Cat# M22426 Wheat-germ agglutinin/Alexa Fluor 555 conjugate Invitrogen Cat# W32464 Concanavalin A/Alexa Fluor 488 conjugate Invitrogen Cat# C11252 Phalloidin/Alexa Fluor 568 Invitrogen Cat# A12380 SYTO 14 green fluorescent nucleic acid stain Invitrogen Cat# S7576 Hoechst 33342 Invitrogen Cat# H3570 Bacterial ribosome This paper N/A Yeast ribosome This paper N/A Human ribosome This paper N/A Plant ribosome This paper N/A Critical Commercial Assays CellTiter Blue Promega Cat# G8080 BioPORTER Protein Delivery Reagent QuickEase Kit AMS Biotechnology (Europe) Cat# BP502424 Deposited Data Morphological profiles and correlation matrix This paper Mendeley Data: https://doi.org/10.17632/ 772b973hhz.1 Phenomycin NMR structure This paper BMRB: 50088 Phenomycin structure This paper PDB: 6TKT (Continued on next page) Structure 28, 1–12.e1–e9, May 5, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines Human: U2OS cell line (taken from 15-year-old female) ATCC ATCC HTB-96; RRID:CVCL_0042 Human: MCF7 (taken from 69-year-old female) ATCC ATCC HTB-22; RRID:CVCL_0031 Human: PANC-1 (taken from 56-year-old male) Cell Line Services (CLS) CLS 300228; RRID:CVCL_0480 Human: BxPC3 (taken from 61-year-old female) Sigma Sigma 93120816; RRID:CVCL_0186 Human: HCC827 (taken from a 39-year-old female) ATCC ATCC CRL-2868; RRID:CVCL_2063 Human: MDA-MB-231 (taken from a 51-year-old female) Cell Line Services (CLS) CLS 300275; RRID:CVCL_0062 Human: A549 (taken from a 58-year-old male) Sigma Sigma 86012804; RRID:CVCL_0023 Human: SiHa (taken from a 55-year-old female) ATCC ATCC HTB-35; RRID:CVCL_0032 Human: HaCat (taken from a 62-year-old male) Creative Bioarray CSC-C8977H; RRID:CVCL_0038 Mouse: AML12 (taken from male) ATCC ATCC CRL-2254; RRID:CVCL_0140 Human: FreeStyleTM HEK-293F (taken from female embryo) Thermo Thermo R79007; RRID:CVCL_6642 Experimental Models: Organisms, Strains S. Cerevisiae - Dstm1: (strain JD1559) J. Dinman, (University of Maryland) N/A Oligonucleotides Fwd K69A CCTGCAAAGGATGCGAAGAGTGATATG This paper N/A Rev K69A CATATCACTCTTCGCATCCTTGCAGG This paper N/A Fwd M3C phenomycin CTTTATTTTCAGGGCGCCTGTGCGAA TCCAAAGACCATC This paper N/A Rev M3C phenomycin GATGGTCTTTGGATTCGCACAGGCGC CCTGAAAATAAAG This paper N/A Fwd M3C aquimarinamycin CTTTATTTTCAGGGCGCCTGTGGACCC ATTAAGGATCGTGAC This paper N/A Rev M3C aquimarinamycin GATCCTTAATGGGTCCACAGGCGCCCT GAAAATAAAGATTC This paper N/A Fwd M3C carotamycin CTTTATTTTCAGGGCGCCTGTGCTATTA GCGATAATGACTACAACCG This paper N/A Rev M3C carotamycin CATTATCGCTAATAGCACAGGCGCCCTG AAAATAAAGATTCTC This paper N/A Recombinant DNA pETM11 EMBL G€unter Stier GFP-Rab7 Choudhury et al., 2002 Addgene plasmid # 12605 Addgene_12605 KSH_AAA (see Table S3) This paper N/A (Continued on next page) e2 Structure 28, 1–12.e1–e9, May 5, 2020

    Staining:

    Article Title: Structure and Function of the Bacterial Protein Toxin Phenomycin.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains DH5a NEB Cat# C2989K BL21 (DE3) NEB Cat# C2527I LOBSTER Andersen et al., 2013 N/A Biological samples Cell pellet from Thermus Thermophilus HB27 BFF, Athens-Gerorgia, USA https://bcmb.franklin.uga.edu/bff N/A Wheat germ Sigma Aldrich Cat# W0125 Chemicals, Peptides, and Recombinant Proteins HisTrapTM Excel column GE Healthcare Cat# GE17-3712-05 Amicon Ultra-4 spin columns Sigma Aldrich Cat# UFC800308 C4 column Vydac Cat# 214TP510 SA matrix Bruker Cat# 8201345 Tris(3- hydroxypropyltriazolylmethyl)amine (THPTA) Sigma Aldrich Cat# 762342 Sulpho-Cy5 azide Lumiprobe Cat# A3330 FAM-azide Lumiprobe Cat# C4130 Azide-PEG3-biotin Sigma Aldrich Cat# 762024 (+)-Sodium L-ascorbate Sigma Aldrich Cat# A7631 Nunc Lab-Tek 8-well chambered coverglass Thermo Scientific Cat# 145411 Poly-D-lysine hydrobromide Sigma Aldrich Cat# P7405 Hoechst 33342 Thermo Scientific Cat# 62249 Lipofectamine 3000 Reagent Kit Thermo Scientific Cat# L3000001 Transferrin Alexa fluor 555 conjugate Invitrogen Cat# T35352 FBS Gibco Cat# A3160802 Dip and Read Streptavidin sensors FortéBio Cat#18-5019 Zeba Spin Desalting Columns, 7 kDa cut-off Thermo Scientific Cat# 89890 MitoTracker Deep Red Invitrogen Cat# M22426 Wheat-germ agglutinin/Alexa Fluor 555 conjugate Invitrogen Cat# W32464 Concanavalin A/Alexa Fluor 488 conjugate Invitrogen Cat# C11252 Phalloidin/Alexa Fluor 568 Invitrogen Cat# A12380 SYTO 14 green fluorescent nucleic acid stain Invitrogen Cat# S7576 Hoechst 33342 Invitrogen Cat# H3570 Bacterial ribosome This paper N/A Yeast ribosome This paper N/A Human ribosome This paper N/A Plant ribosome This paper N/A Critical Commercial Assays CellTiter Blue Promega Cat# G8080 BioPORTER Protein Delivery Reagent QuickEase Kit AMS Biotechnology (Europe) Cat# BP502424 Deposited Data Morphological profiles and correlation matrix This paper Mendeley Data: https://doi.org/10.17632/ 772b973hhz.1 Phenomycin NMR structure This paper BMRB: 50088 Phenomycin structure This paper PDB: 6TKT (Continued on next page) Structure 28, 1–12.e1–e9, May 5, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines Human: U2OS cell line (taken from 15-year-old female) ATCC ATCC HTB-96; RRID:CVCL_0042 Human: MCF7 (taken from 69-year-old female) ATCC ATCC HTB-22; RRID:CVCL_0031 Human: PANC-1 (taken from 56-year-old male) Cell Line Services (CLS) CLS 300228; RRID:CVCL_0480 Human: BxPC3 (taken from 61-year-old female) Sigma Sigma 93120816; RRID:CVCL_0186 Human: HCC827 (taken from a 39-year-old female) ATCC ATCC CRL-2868; RRID:CVCL_2063 Human: MDA-MB-231 (taken from a 51-year-old female) Cell Line Services (CLS) CLS 300275; RRID:CVCL_0062 Human: A549 (taken from a 58-year-old male) Sigma Sigma 86012804; RRID:CVCL_0023 Human: SiHa (taken from a 55-year-old female) ATCC ATCC HTB-35; RRID:CVCL_0032 Human: HaCat (taken from a 62-year-old male) Creative Bioarray CSC-C8977H; RRID:CVCL_0038 Mouse: AML12 (taken from male) ATCC ATCC CRL-2254; RRID:CVCL_0140 Human: FreeStyleTM HEK-293F (taken from female embryo) Thermo Thermo R79007; RRID:CVCL_6642 Experimental Models: Organisms, Strains S. Cerevisiae - Dstm1: (strain JD1559) J. Dinman, (University of Maryland) N/A Oligonucleotides Fwd K69A CCTGCAAAGGATGCGAAGAGTGATATG This paper N/A Rev K69A CATATCACTCTTCGCATCCTTGCAGG This paper N/A Fwd M3C phenomycin CTTTATTTTCAGGGCGCCTGTGCGAA TCCAAAGACCATC This paper N/A Rev M3C phenomycin GATGGTCTTTGGATTCGCACAGGCGC CCTGAAAATAAAG This paper N/A Fwd M3C aquimarinamycin CTTTATTTTCAGGGCGCCTGTGGACCC ATTAAGGATCGTGAC This paper N/A Rev M3C aquimarinamycin GATCCTTAATGGGTCCACAGGCGCCCT GAAAATAAAGATTC This paper N/A Fwd M3C carotamycin CTTTATTTTCAGGGCGCCTGTGCTATTA GCGATAATGACTACAACCG This paper N/A Rev M3C carotamycin CATTATCGCTAATAGCACAGGCGCCCTG AAAATAAAGATTCTC This paper N/A Recombinant DNA pETM11 EMBL G€unter Stier GFP-Rab7 Choudhury et al., 2002 Addgene plasmid # 12605 Addgene_12605 KSH_AAA (see Table S3) This paper N/A (Continued on next page) e2 Structure 28, 1–12.e1–e9, May 5, 2020

    Nuclear Magnetic Resonance:

    Article Title: Structure and Function of the Bacterial Protein Toxin Phenomycin.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains DH5a NEB Cat# C2989K BL21 (DE3) NEB Cat# C2527I LOBSTER Andersen et al., 2013 N/A Biological samples Cell pellet from Thermus Thermophilus HB27 BFF, Athens-Gerorgia, USA https://bcmb.franklin.uga.edu/bff N/A Wheat germ Sigma Aldrich Cat# W0125 Chemicals, Peptides, and Recombinant Proteins HisTrapTM Excel column GE Healthcare Cat# GE17-3712-05 Amicon Ultra-4 spin columns Sigma Aldrich Cat# UFC800308 C4 column Vydac Cat# 214TP510 SA matrix Bruker Cat# 8201345 Tris(3- hydroxypropyltriazolylmethyl)amine (THPTA) Sigma Aldrich Cat# 762342 Sulpho-Cy5 azide Lumiprobe Cat# A3330 FAM-azide Lumiprobe Cat# C4130 Azide-PEG3-biotin Sigma Aldrich Cat# 762024 (+)-Sodium L-ascorbate Sigma Aldrich Cat# A7631 Nunc Lab-Tek 8-well chambered coverglass Thermo Scientific Cat# 145411 Poly-D-lysine hydrobromide Sigma Aldrich Cat# P7405 Hoechst 33342 Thermo Scientific Cat# 62249 Lipofectamine 3000 Reagent Kit Thermo Scientific Cat# L3000001 Transferrin Alexa fluor 555 conjugate Invitrogen Cat# T35352 FBS Gibco Cat# A3160802 Dip and Read Streptavidin sensors FortéBio Cat#18-5019 Zeba Spin Desalting Columns, 7 kDa cut-off Thermo Scientific Cat# 89890 MitoTracker Deep Red Invitrogen Cat# M22426 Wheat-germ agglutinin/Alexa Fluor 555 conjugate Invitrogen Cat# W32464 Concanavalin A/Alexa Fluor 488 conjugate Invitrogen Cat# C11252 Phalloidin/Alexa Fluor 568 Invitrogen Cat# A12380 SYTO 14 green fluorescent nucleic acid stain Invitrogen Cat# S7576 Hoechst 33342 Invitrogen Cat# H3570 Bacterial ribosome This paper N/A Yeast ribosome This paper N/A Human ribosome This paper N/A Plant ribosome This paper N/A Critical Commercial Assays CellTiter Blue Promega Cat# G8080 BioPORTER Protein Delivery Reagent QuickEase Kit AMS Biotechnology (Europe) Cat# BP502424 Deposited Data Morphological profiles and correlation matrix This paper Mendeley Data: https://doi.org/10.17632/ 772b973hhz.1 Phenomycin NMR structure This paper BMRB: 50088 Phenomycin structure This paper PDB: 6TKT (Continued on next page) Structure 28, 1–12.e1–e9, May 5, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines Human: U2OS cell line (taken from 15-year-old female) ATCC ATCC HTB-96; RRID:CVCL_0042 Human: MCF7 (taken from 69-year-old female) ATCC ATCC HTB-22; RRID:CVCL_0031 Human: PANC-1 (taken from 56-year-old male) Cell Line Services (CLS) CLS 300228; RRID:CVCL_0480 Human: BxPC3 (taken from 61-year-old female) Sigma Sigma 93120816; RRID:CVCL_0186 Human: HCC827 (taken from a 39-year-old female) ATCC ATCC CRL-2868; RRID:CVCL_2063 Human: MDA-MB-231 (taken from a 51-year-old female) Cell Line Services (CLS) CLS 300275; RRID:CVCL_0062 Human: A549 (taken from a 58-year-old male) Sigma Sigma 86012804; RRID:CVCL_0023 Human: SiHa (taken from a 55-year-old female) ATCC ATCC HTB-35; RRID:CVCL_0032 Human: HaCat (taken from a 62-year-old male) Creative Bioarray CSC-C8977H; RRID:CVCL_0038 Mouse: AML12 (taken from male) ATCC ATCC CRL-2254; RRID:CVCL_0140 Human: FreeStyleTM HEK-293F (taken from female embryo) Thermo Thermo R79007; RRID:CVCL_6642 Experimental Models: Organisms, Strains S. Cerevisiae - Dstm1: (strain JD1559) J. Dinman, (University of Maryland) N/A Oligonucleotides Fwd K69A CCTGCAAAGGATGCGAAGAGTGATATG This paper N/A Rev K69A CATATCACTCTTCGCATCCTTGCAGG This paper N/A Fwd M3C phenomycin CTTTATTTTCAGGGCGCCTGTGCGAA TCCAAAGACCATC This paper N/A Rev M3C phenomycin GATGGTCTTTGGATTCGCACAGGCGC CCTGAAAATAAAG This paper N/A Fwd M3C aquimarinamycin CTTTATTTTCAGGGCGCCTGTGGACCC ATTAAGGATCGTGAC This paper N/A Rev M3C aquimarinamycin GATCCTTAATGGGTCCACAGGCGCCCT GAAAATAAAGATTC This paper N/A Fwd M3C carotamycin CTTTATTTTCAGGGCGCCTGTGCTATTA GCGATAATGACTACAACCG This paper N/A Rev M3C carotamycin CATTATCGCTAATAGCACAGGCGCCCTG AAAATAAAGATTCTC This paper N/A Recombinant DNA pETM11 EMBL G€unter Stier GFP-Rab7 Choudhury et al., 2002 Addgene plasmid # 12605 Addgene_12605 KSH_AAA (see Table S3) This paper N/A (Continued on next page) e2 Structure 28, 1–12.e1–e9, May 5, 2020



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